Coding

Part:BBa_K2668010:Design

Designed by: Younes Bouchiba   Group: iGEM18_Toulouse-INSA-UPS   (2018-08-16)


Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 358
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part BBa_K2668010 comes from the iGEM Team Toulouse-INSA-UPS Cerberus Project. It was originally designed to bind a variety of compounds to cellulose thanks to its two linkers. The streptavidin linker can bind biotin and biotinylated compounds while the UnAA-containing linker will click molecules that bear an alkyne function.


Source

The Part was obtained by IDT gBlock synthesis. The CBM3a domain is a corrected version of the Part:BBa_K1321014, RFC10 compatible. The monomeric streptavidin is the Part:BBa_K1896000.


References